Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). The next day, precultures were diluted to an optical density of 0. The papers, which were published between 2010 and. The samples were transferred to black 96-well plates and fluorescence was measured at an excitation wavelength of 405 nm and an emission wavelength of 535 nm on the FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices) equipped with the SoftMaxPro Microplate Data Acquisition and Analysis 6. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $3,500. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Place plate in the GloMax® Instrument and read. 4 Pages. FilterMax F3 & F5 Microplate Readers. Add to cart. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. 3 Pages. The. Vascular. 4 Pages. Optical System: High energy xenon flash lamp. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. 05); b Evaluation of the ATP levels of T. 40266 Email: drugdiscoverytech@lifetech. This reader provides visible absorbance. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. Bioprocess Equipment and Technology. The Hidex Sense is a compact, state-of-the-art, multi-mode microplate reader which provides a powerful and flexible platform for performing any kind of assay without limitations. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. com B. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. Click Run. Intended Use Statement The Synergy H1 is a hybrid multi-mode microplate reader. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). Measurements of Caspase-3/7 Activity. Renilla/Firefly luciferase activities were measured twice. Supplier: Molecular Devices. Please note it is NOT a Keyspan USA-19 HS USB adaptor that is commonly used by. cruzi incubated for 1 h with sertraline at 2 μM. Manufacturer: Molecular Devices. 00 USD. The cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. 8 Pages. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. shopping cart 0 items $0. 425 or. ** Some compatible filter sets are available for purchase from Life. 2. ” e. 0´ 1 4 4. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). – ™FilterMax F5 Multi-Mode Microplate Reader (cat. Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. A FilterMax F5 multi-mode microplate reader (Molecular Devices, San Jose, USA) was used to determine the absorbance (OD 450nm -OD 570nm). 50/50. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. SpectraMax Glo Steady-Luc Reporter Assay Kit. ScanLater Western Blot Detection System. Z’-LYTE® Compatible Microplate Reader Documentation Version No. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Plaque reduction. 3 Pages. 2 Pages. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. FilterMax F5 Multi-Mode Microplate Reader. 40266 Email: [email protected]™ F3 and F5 Multimode Microplate Readers, Molecular Devices. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1. F5 Multi-Mode Microplate Reader. The drawing shows a standard microplate of 15 mm height covered with a lid. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Vertical Jump. Caspase-3 Activity. The fluorescent signals were measured at 10 minutes and one hour after the Stoplight Red substrateFilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) 12. They provide absorbance, fluorescence, glow luminescence, and other read modes for. Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. ScanLater Western Blot Detection System. 5. MetaMorph NX. Transfluor Technology. CloneMatrix. Sunnyvale, Calif. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. Different parameters (for instance, the incubation time of bacteriophage-MBs and S. Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set. Protein quantification was carried out using the Pierce™ BCA Protein Assay Kit (Thermo Fisher) and a FilterMax F5 Multi-Mode Microplate Reader (San Jose, CA, United SA). * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Owner, Zeckhausen. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. 09 CAD. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Your applications, your modes, your choice. The values of luminescence are directly proportional to the number of viable cells. Close Search. Microplate readers. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Mazumder, in Laser Additive Manufacturing, 2017 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. 003 fmol 0. Each condition was performed in. Plate Formats:. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The amount of colorimetric product was continuously monitored for 120 min. 713579). shopping cart 0 items $0. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Description: The FilterMax™ F3 features absorbance (visible),. HERM LB 500 with NaI detector Radio HPLC Detector. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Products : General, FilterMax F5 reader. The 50% inhibitory concentration (IC 50) values were determined using the nonlinear regression curve fit analysis of Prism 9. 3 and higher include optimized reader settings, as well as data analysis, for faster results. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). 2. Manufacturer: Molecular Devices Model: FilterMax F5 Condition: Used. F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . SpectraMax Glo Steady-Luc Reporter Assay Kit. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). 00 USD. Please click on the links below to view the Microplate Reader Comparison Tables. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. shopping cart 0 items $0. Multi-Mode Microplate Reader. 1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices). Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Zebrafish whole-eye mRNA extraction and RT-PCRSearch LabX. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). 3 Pages. CloneMatrix. ScanLater Western Blot Detection System. FilterMax F5 Multi-Mode Microplate Reader. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. ] Example of document to evaluate if the reader . 3 Pages. 00. After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. The quantity of the colorimetric product was monitored continuously for 120 min. F5 has made an exception to this policy for BIG-IP 13. FilterMax F5 Multi-Mode Microplate Reader. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. 3. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $ 3,250. 8 Pages. 4 Pages. 0´ 104 6. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. 3 Pages. In . Delete from my manuals. 2 Temperature and cooling rate control. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 100 fmol 1536-well (8 µL) 0. A 1:1 binding ratio Hill model was used to fit the binding interaction data. FilterMax F5 Multi-Mode Microplate Reader. Transfluor Technology. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. 3 Pages. Filters: Excitation and emission slides for 4 filters each. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. F3/F5 Multi-Mode Microplate Readers. Detection modalities are shown in Table 1-1. The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). 4 Pages. Download Table of Contents. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Briefly, serial dilutions of TNP were incubated with 200 TCID 50 virus in presence of diethylaminoethyl-dextran and the neutralizing activity was assessed by measuring luciferase activity after 48 h using a FilterMax F5 multi-mode microplate reader (Molecular Devices). Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. 5. The 0. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. VANTAstar™ Flexible Multi-mode Microplate Reader. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. 2 Pages. 3. 2. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#: STEM-LE-0509-LC) Mithras LB 940 Multimode Microplate Reader, 200-1000 nm Spectral Range Absorbance, BERTHOLD TECHNOLOGIES GmbH & Co. IMAP assays are based on binding of. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. 5 ml 0. DMSO was used to dissolve the compounds and was included in the microplate at 0. A Vision for the Future With TTP LabTech. FilterMax F3 & F5 Multi-Mode Microplate Readers. Multi-Mode microplate readers offer flexibility and ease of use over a broad range of applications. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 6% from 2020 to 2027. CloneMatrix. Upgradeable modules are also available including western blot, cell imaging, and fast. ** Some compatible filter sets are available for. The microplate drawer door must be held open manually while. A blank sample was prepared by mixing. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. MetaFluor Software Brochure. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). Other technologies such as imaging. 00. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Select the protocol “Concentration_pico_green_v3. 3 Pages. 00. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Page: 1/4 NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. 00 USD. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax QuickDrop UV-Vis Spectrophotometer. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 666 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. 3. performs within its specifications. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. Transfluor Technology. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. book Page 1 Tuesday, September 7, 2010 6:14 PM. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. SpectraMax Quant dsDNA Assay Kits. FilterMax F5 Multi-Mode Microplate Reader. The donor and acceptor can be used to label a variety of biomolecules, for. ” 3. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. 4 million by 2027; it is expected to grow at a CAGR of 3. Applications: Absorbance, Cell viability, ELISA, Fluorescence,. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. com A. The OD 595 of the culture in the plate (light path length: ∼6. 00The microplate reader market was valued at US$ 534. FilterMax F5 Multi-mode microplate reader. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. GPCRs. MetaMorph NX. , Sunnyvale, CA, USA) and normalized to the absorbance in the. 4 Pages. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. SpectraMax Glo Steady-Luc Reporter Assay Kit. SpectraMax Gemini XPS/EM Microplate Readers. A 1:1 binding ratio Hill model was used to fit the binding interaction data. View specifications, prices, citations, reviews, and more. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). The ucs load command creates a backup of. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. Delete from my manuals. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. 3 Pages. FilterMax Multi-Mode Microplate Readers Functionality FilterMax F3 FilterMax F5 Absorbance (ABS ) Visible 4 4 Absorbance (ABS) UV 4 Fluorescence intensity (FI) top read 4 4 Fluorescence intensity (FI) bottom read 4 Luminescence (LUM) glow 4 4 Fluorescence polarization (FP) 4 Time-Resolved fluorescence (TRF) 4 Shaking 4 4 Temperature. S. Load prepared PicoGreen plate (step C. The OD 600 was plotted against time using Graphpad Prism 8. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. SpectraMax iD5. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. • H1_M_FI_T_MUB. Buffer without protease was used as a blank for background subtraction. Align the two slots on the bottle holder with the two pegs on the rail. Neurotransmitter Transporter Uptake Assay Kit. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 3 Pages. Devices FilterMax Multi-Mode Microplate Readers. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. In addition, the instrument features linear and orbital shaking, plus temperature control. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. 2 μM ATP standard was. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. Easily accessible six-position filter slides enable wavelength selection from 340 to 650nm. The following document is. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. 01% in 2030. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. 8. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. FilterMax F3 & F5 Microplate Readers. FLIPR Membrane Potential Assay Kits. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Internal controls were. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. 6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. 1 (GraphPad Software Inc. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. MIA PaCa-2 cells were cultured in 96-well plates with 1000 cells/well. Methods for MycoAlert and MycoAlert PLUS Assays are basically the same, except for the concentration range of assay control used, and are detailed below. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. MetaFluor Software. In addition, the instrument features linear and orbital shaking, plus temperature. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Category 2 and 3 microplate readers use gradient heating as well. Page 2 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 7. 3 Pages. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. SoftMax® Pro Microplate Data Analysis Software provides integrated instrument control and data analysis. Figure 1. Watson. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). The standard curve presented a linear phase from 1. 2 Pages. Topic. Important things to consider when purchasing a multimode microplate reader. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. The OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). Sign In Upload. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. Plate Formats:. State-of-the-art Multi-Mode Microplate Reader. Instrument Setup: 1. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 3 Pages. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Figure 1: Schematic of a typical workow of the semi solid cloning process. 6 reviews. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 100 fmol 384-well (75 µL) 0. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. 3 Pages. 4 Pages. 8 Pages. 012 fmol 0. the luminescence reading was taken on a FilterMax F5 Multi-Mode. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. 3. 425 or. Add to cart. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched value and flexibility for a variety of applications capable of performing: - Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. Transfluor Technology. Microplate Readers.